Vitamin B12 1000

What Methylcobalamin Is

Vitamin B12 (cobalamin) is a water-soluble vitamin that mammals cannot synthesise. Milo-Lab supplies it as methylcobalamin, a coenzyme form with a methyl group bound to the central cobalt atom, at 1000mcg/ml in a 10ml vial.

Enzyme Cofactor Roles

• Methionine synthase: homocysteine remethylation to methionine (methylcobalamin)
• Methylmalonyl-CoA mutase: conversion of methylmalonyl-CoA to succinyl-CoA in mitochondria (adenosylcobalamin)
• Methyl-group supply for DNA, RNA and neurotransmitter biosynthesis

How B12 Works

B12 acts as a cofactor for two enzymes. As methylcobalamin it is the cofactor of methionine synthase, converting homocysteine back to methionine and feeding the methyl groups used in DNA, RNA and neurotransmitter synthesis. As adenosylcobalamin it is the cofactor of methylmalonyl-CoA mutase in the mitochondria, a step that channels odd-chain fatty acids and branched-chain amino acids into the citric-acid cycle via succinyl-CoA. Methylcobalamin is already a coenzyme form and does not require the conversion step that cyanocobalamin does.

Storage and Handling

Storage: keep refrigerated at 2-8°C and protect from light, as B12 is light-sensitive. Do not freeze. Handling: supplied as a solution. Return the vial to refrigeration after each opening.

For research purposes only. Not for human consumption.